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KMID : 0438219740110021207
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Korea University Medical Journal
1974 Volume.11 No. 2 p.1207 ~ p.1220
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An Experimental Study on the Effect of X Rays and Cortisone on Erlich Ascites Tumor in the Mouse
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Abstract
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In an attempt to evaluate the effects of cortisone and whole-body irradiation on immune response and rejection phenoma, the author made an experimental observation on the changes in the.-peripheral blood, ascites and peritoneal tissues of the inbred mouse following transplantation of Erlich Acites Tumor. Two-hundred-and-two mices of SM strain were divided into following groups;
1) Normal control group: 10 animals
2) Experimental groups: 72
(1) Tumor-transplanted control group
Only Erlich ascites tumor cells (1X106) were inoculated into the peritoneal cavity.
(2) Irradiated control group
A single dose of 400 R x rays was given to the whole body.
(3) Animal group given a single dose of cortisone
A single dose of 2.5 mg cortisone acetate was injected subcutaneously.
(4) Animal group given multiple doses of cortisone
Four injections of 2.5 mg cortisone were done at an interval of 24 hours.
3) Experimental tumor-transplanted groups: 120
(1) Animal group irradiated before tumor transplantation
A single dose of 400 R x rays was given to the whole body 15 hours before tumor transplantation.
(2) Animal group irradiated after tumor implantation
A single dose of 400 R x rays was given to the whole body 24 hours after tumor transplantation.
3) Tumor-transplanted group pretreated with a single dose of cortisone
A single dose of 2.5 mg cortisone was injected 24 hours prior to tumor transplantation.
4) Tumor-transplanted group treated with multiple doses of cortisone
A single dose of 2.5 mg cortisone was given 24 hours before tumor transplantation followed by 3 more injections of 2.5 mg cortisone at 24-hour intervals.
The results obtained are summarized as follows:
1. There was significant change in total leukocyte and lymphocyte counts in the peripheral blood of tumor transplanted groups, with steady progressive increase in number of white blood cells after 7th day.
2. Rejection response appeared prominently at 7th day in the tumor-transplanted control group and progressed rapidly. After the 7th day leukocytes, especially lymphocytes, increased rapidly, the tumor cells in the ascitic fluid markedly decreased in number and the histological picture of the peritoneal tissues revealed progressive tumor necrosis and lymphocytic infiltration. Although rejection phenomenon manifested around the same 7th day, it was considerably mild and much slower in the tumor-transplanted group pretreated with k rays or cortisone.
3. Cortisone-treated groups
Rejection response was significantly suppressed with a single dose of cortisone. Not only tumor cells were actively growing but there was no apparent lymphocytic infiltration or hemorrhage in the peritoneal tissues until the end of 2nd week. In the tumor implanted group treated with 4 doses of cortisone, the histologic findings were identical with those of the single dose group, but the time when leukocytes begin to increase and ascitic tumor cells fall off in number was deferred by 5 days, and rebound phenomenon was evident.
4. Irradiated groups
Rejection response was least prominent when whole-body irradiation was done 15 hours before tumor transplantation while no significant suppressive effect could be obtained when a sublethal dose of x rays was given 24 hours after tumor implantation.
The ascitic fluid was invariably bloody in the irradiated groups.
5. The number of leukocytes in the peripheral blood and that of tumor cells in the ascitic fluid changed inversely each other.
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